PIC-BIO,Inc. - We aim at the establishment of health and the safety of poultry and livestock product.


SALTOSE is for treatment of infections caused by Clostridium, Salmonella, Coccidiosis, Cryptosporidiosis with our own-developed enzyme called "Cell Wall Lyaze".

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New Probiotics & Strong Enzyme Combination

Our Bacillus bacteria which are screened among 10,000 strains and special enzymes ensure the safety and health of poultry and livestock by controlling harmful bacteria (Salmonella, E. coli, Clostridium) and parasites (Coccidium, Cryptosporidium)

PIC-BIO INC. has been involved in research and development of remedies for not only production of antibiotic-free poultry and livestock but also enzyme products that are capable of preventing migration of zoonotic diseases as replacement of antibiotic for number of years since PIC-BIO INC. was incorporated.
One of the enzyme products is "SALTOSE" by its trade name whose efficacy has been recognized by poultry and livestock industries in Japan and foreign countries.

What is SALTOSE?

SALTOSE is one of leading probiotics which contains Bacillus group, Enterococcus group and unique enzymes. SALTOSE can prevent and treat the bacterial and parasitic diseases with the characteristics of used bacteria and enzymes, and improve the productivity for poultry and livestock.

Ingredients and Characteristics

We have selected 5 strains of Bacillus group among more than ten thousand strains of Bacillus group which do not produce antibiotics, but produce unique enzymes which can break down the cell wall of bacteria and oocyst of some important parasites.

The Bacillus group;
1.Bacillus licheniformis
2.Bacillus subtilis
3.Bacillus pumilus
  1. Disease Control
    Strong bacteriolytic effects against bacterial diseases and antiprotozoal effect against parasitic diseases through unique enzyme“ Cell-Wall Lyase”.
  2. Improvement in Digestion
    Disintegration of the cell wall of feed substances for improvement of productivity by enhancing digestion, feed conversion, and body weight gain.
  3. Odor Control
    Decrease of Ammonia and Hydrogen Sulfide in their excreta.
The main enzymes;
2.Cell-Wall Lyase

The Enterococcus group;

1.Enterococcus faecalis

2.Enterococcus faecium

  1. Improving ecological environment in intestines
  2. Enhancing multiplication and growth of bifido bacteria
  3. Prevention and elimination of harmful bacteria
  4. Keeping microorganisms in rumen and caecum at normal conditions
  5. Enhancing appetite of animals and also enhance their growth


Lysis Effects on Eggs of Parasites or Oocysts 2 - 1

Coccidium oocyst (Poultry)



Coccidium oocyst germinated and sporocyst developed

Coccidium oocyst was dissolved completely and its original shape disappeared.

Coccidium oocyst (Cattle)



Coccidium oocyst germinated and sporocyst developed.

Coccidium oocyst was dissolved completely and its original shape disappeared.

Lysis Effects on Eggs of Parasites or Oocysts 2 - 2

Cryptosporidium (Cattle)

Prepared culture media for SALTOSE and inoculated Cryptosporidium for observation of degradation effects.

Before SALTOSE application

After 12 hours
* Blue stick bacteria are SALTOSE bacteria

Finally, Cryptosporidium was dissolved completely and its original shape disappeared.

Inhibitory Effects on Bacteria 2 - 1

Salmonella Enteritidis

Prepared culture media for S.Entertidis and inoculated the follwing bacteria.

1, 6, 7, 8, 9 : SALTOSE
T : Competitor
C : Competitor
S1 : S.Typhimurium
S2 : S.Gallinarum

SALTOSE shows their antibacterial activities with the formation of inhibition ring.

Salmonella Enteritidis and Salmonella Gallinarum

RL bacteria were inoculated in YM-SE and YM-SG culture medium for observation of inhibition ring.

YM : Yeast and Malt
RL : SALTOSE bacteria
SE : S.Enteritidis
SG : S.Gallinarum
SP : other Bacillus subtilis
  1. SALTOSE produced a large inhibition ring, which indicates strong control effect on S.Enteritidis and S.Gallinarum
  2. Other Bacillus subtilis indicated almost no control effect.

Inhibitory Effects on Bacteria 2 - 2


Prepared culture medium for Clostridium and inoculated Bacillus licheniformis of SALTOSE (Right side) and the other Bacillus licheniformis (Left side) bacteria for observation of growth and inhibition ring.

12 hours

32 hours

60 hours

Graph for Right side
(Dimension (㎠) of bacteria and inhibition ring)

32 hours after the inoculation, Bacillus licheniformis of SALTOSE stopped their growth due to completing the absorption of nutrients from culture medium.
However, inhibition ring continued to expand, and dissolved Clostridium.

Antagonistic Effects on Bacteria 3 - 1

Salmonella Enteritidis(840) and Salmonella Infantis (837)



Competitor did not absorb the inoculated Salmonella bacteria. SALTOSE absorbed the inoculated Salmonella bacteria.

Antagonistic Effects on Bacteria 3 - 2

Salmonella Gallinarum

S.Gallinarum were inoculated in DP (Left side) and YM (Right side) culture medium, and then, each bacterium was inoculated in the same point for observation of antagonistic effects.

DP : Dexton and Pepton
SE : S.Enteritidis
RL : SALTOSE bacteria
SP : Other Bacillus subtilis
YM : Yeast and Malt
SG : S.Gallinarum
  1. The growth power of SALTOSE bacteria is the strongest, and SALTOSE bacteria controls S.Gallinarum bacteria.
  2. Other Bacillus subtilis indicated poor growth power.

Antagonistic Effects on Bacteria 3 - 3

E. coli (K-88)

E. coli (K-88) and SALTOSE bacteria were inoculated on the same culture medium. It is observed that SALTOSE shows more antibacterial activities against E. coli .

Useful Bacteria (Lactic acid bacteria and Bifido bacteria)

Lactic acid bacteria

Bifido bacteria

SALTOSE did not damage the useful bacteria.

The Other Data

Testing result (Excreta of poultry)

1. Reduction of generating ammonium odor
Generation of ammonia from broiler farm was reduced due to application of SALTOSE.
2. Comparison of digestibility

C: Control

Result: SALTOSE treatment can improve the digestion, so the amount of undigested materials in the excreta becomes half as compared with the control.

3. Bacterial 1 count in the excretion of chickens

The count of Bacillus bacteria in the excretion remained almost unchanged; however, the total bacteria count was reduced as dosage rate was increased.